Figure 6. Phosphodiesterase inhibitors restore the ethanol suppression of adenosine-induced transepithelial chloride conductance.

The air-liquid interface cultures of Calu-3 cells were exposed to 0 and 100 mM of ethanol for 24 hours. Adenosine-stimulated I_SC was measured, showing that ethanol-exposed Calu-3 epithelia had significantly lower adenosine-stimulated I_SC than the no ethanol control. However, phosphodiesterase inhibitors, IBMX (100 µM) or papaverine (50 µM), fully restored the ethanol-suppressed adenosine-stimulated I_SC. Asterisks indicate statistically significant differences between groups (p<0.05, n = 4 for each group).