Figure 5. PGH₁ induces eosinophil adhesion to human pulmonary microvascular endothelial cells under flow conditions.

Eosinophils were pre-incubated with vehicle (A–C) or 10 µM CRTH2-specific antagonist TM30089 (D–F) for 10 min at room temperature followed by treatment with vehicle (A, D), 1 µM PGH₁ (B, E) or 30 nM PGD₂ (C, F) for 10 min at 37°C. Eosinophils were then superfused over human pulmonary microvascular endothelial cells grown on VenaEC biochips (Cellix, Dublin) for 5 min at 37°C. Representative images were taken 5 min after start of the superfusion (A–F). G: averaged data from A–F, quantified by computerized image analysis. Data are shown as mean + SEM of 4 experiments. *P<0.05 PGD₂ versus TM30089+PGD₂ and PGH₁ versus TM30089+PGH₁.