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. 2012 Mar 19;7(3):e33786. doi: 10.1371/journal.pone.0033786

Figure 1. Yeast RRS screen with necdin as bait.

Figure 1

Representative pictures of yeast cdc25-2 colonies after transformation with different candidates at the permissive temperature (24°C), and at the restrictive temperature (36°C). All colonies grow on galactose medium at 24°C, while only colonies expressing candidates that interact with the bait can grow on galactose at 36°C, but not on YPD (prey candidates are under a galactose promoter).