Figure 1. Yeast RRS screen with necdin as bait.
Representative pictures of yeast cdc25-2 colonies after transformation with different candidates at the permissive temperature (24°C), and at the restrictive temperature (36°C). All colonies grow on galactose medium at 24°C, while only colonies expressing candidates that interact with the bait can grow on galactose at 36°C, but not on YPD (prey candidates are under a galactose promoter).