Table 1. Fractions of cells obtained before and after sorting on day 1.
Cell population | Day seeded | Day analyzed | % positive cells by immunocytochemistry | % positive cells by FACS | |||
Amylase | CK19 | Vimentin | CD90 | Ca19-9 | |||
Unsorted | 0 | 0 | 61±16b | 6 ±3 | 12±6 | N/A | N/A |
Unsorted | 0 | 1 | 47±17 | 22±6a | 11±4a | 8±7a | 54±16 |
CD90− | 0 | 1 | 42±10 | 18±6a | 8±2a | 0.06±0.01a | N/A |
CD90+ | 0 | 1 | 19±5 | 12±6a | 42±18a | 26±10a | N/A |
Ca19-9+ | 0 | 1 | 16±8 | 51±21a | 2±2a | N/A | 96±2 |
Ca19-9− | 0 | 1 | 72±14 | 5±2a | 24±9a | N/A | 14±13 |
Unsorted | 0 | 8 | 0.6c | 32±7 | 18±11 | 48±18 | 46±20 |
Unsorted | 1 | 8 | 0.5c | 36±10 | 21±5 | 27±9a | 60±11a |
CD90− | 1 | 8 | 2.3c | 59±4 | 10±2 | 0.7±0.4a | 79±8a |
CD90+ | 1 | 8 | 7.4c | 16±3 | 54±21 | 84±3 | 14±1b |
Ca19-9+ | 1 | 8 | 0.6c | 55±10 | 7±2 | 0.7±0.2b | 93±4b |
Ca19-9− | 1 | 8 | 0.5c | 25±10 | 48±18 | 67±9b | 4±1b |
Note: unless otherwise indicated, the number of replicates is N = 3 pancreata.
Number of replicate pancreata = 4.
Number of replicate pancreata = 2.
Number of replicate pancreata = 1.
N/A = non-available.