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. 2012 Mar 19;7(3):e33999. doi: 10.1371/journal.pone.0033999

Table 1. Fractions of cells obtained before and after sorting on day 1.

Cell population Day seeded Day analyzed % positive cells by immunocytochemistry % positive cells by FACS
Amylase CK19 Vimentin CD90 Ca19-9
Unsorted 0 0 61±16b 6 ±3 12±6 N/A N/A
Unsorted 0 1 47±17 22±6a 11±4a 8±7a 54±16
CD90− 0 1 42±10 18±6a 8±2a 0.06±0.01a N/A
CD90+ 0 1 19±5 12±6a 42±18a 26±10a N/A
Ca19-9+ 0 1 16±8 51±21a 2±2a N/A 96±2
Ca19-9− 0 1 72±14 5±2a 24±9a N/A 14±13
Unsorted 0 8 0.6c 32±7 18±11 48±18 46±20
Unsorted 1 8 0.5c 36±10 21±5 27±9a 60±11a
CD90− 1 8 2.3c 59±4 10±2 0.7±0.4a 79±8a
CD90+ 1 8 7.4c 16±3 54±21 84±3 14±1b
Ca19-9+ 1 8 0.6c 55±10 7±2 0.7±0.2b 93±4b
Ca19-9− 1 8 0.5c 25±10 48±18 67±9b 4±1b

Note: unless otherwise indicated, the number of replicates is N = 3 pancreata.

a

Number of replicate pancreata = 4.

b

Number of replicate pancreata = 2.

c

Number of replicate pancreata = 1.

N/A = non-available.