Figure 4. Immunofluorescence staining of CD45 in the cryosections at 4 h: very few CD45+ cells were observed after PBS applications (A), the strongest expression of CD45+ cells in CALT follicles was found after instillation of 0.02%BAK (B), ^0.02%BAK+latanoprost (C) and ^0.015%BAK+travoprost (D).

Scattered CD45+ cells were also found after the instillation of ^0.005%BAK+bimatoprost (E), ^BAK-freetravoprost Z (F) or ^BAK-freetafluprost (G). (Bar, 100 µm). CD45+ cell counts in cryosections are presented in Figure 4H. * P<0.0001 compared to PBS, P<0.001 compared to ^0.005%BAK+bimatoprost, and P<0.0002 compared to ^BAK-freetravoprost Z or ^BAK-freetafluprost.