Skip to main content
. Author manuscript; available in PMC: 2012 Mar 19.
Published in final edited form as: J Mol Endocrinol. 2011 Nov 21;47(3):299–313. doi: 10.1530/JME-11-0060

Figure 2.

Figure 2

Distal and proximal regulatory elements in the HAMP promoter are crucial for its high basal activity in hepatocytes. Huh7 cells were transfected with 1580 ng/well of either a wild-type (WT, −1234/+73) or a mutated HAMP promoter constructs (mutants, M21–M28 in A and M2–M11 in B) along with 20 ng of phRL-TK (Renilla Luciferase) as an internal control. Tri-nucleotide mutations were introduced within the distal site −932/−878 (A) and proximal site −155/−96 (B) promoter regions of the full-length promoter (−1234/+73). In each case the WT HAMP promoter sequence is shown below the x-axis in upper case while the corresponding mutated tri-nucleotides are in lowercase. At least three experiments were done in triplicate using three different DNA preparations for each construct. Results are the relative activity of the constructs (mean±S.E.M., gray bars) with respect to the WT promoter fragment −1234/+73 (black bars). A different superscript indicates a difference between means (a–fP<0·05).