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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Cell Tissue Res. 2012 Jan 26;347(3):689–699. doi: 10.1007/s00441-011-1297-0

Fig. 3.

Fig. 3

Characterization of the cell culture parameters that affect transfection efficiency when hMSCs were treated with 1μg pDNA complexed with 60kDa PEI at an N/P ratio of 7. a) Normalized transfection efficiency of passage 6 hMSCs seeded at a density of 5-5*104 hMSCs/cm2. * Denotes significant difference from 5*102 hMSCs/cm2 (t test, p<0.05). b) Normalized transfection efficiency of passage 6–8 hMSCs. * Denotes significant difference from passage 6 hMSC’s (t test, p<0.05). c) Normalized growth rate of transfected passage 6–8 hMSCs. * Denotes significant difference from passage 6 hMSCs (t test, p<0.05). d) Normalized cell number of hMSCs that were not treated, treated with mitomycin C, or treated with gamma radiation. * Denotes significant difference from untreated hMSCs (t test, p<0.05). e) Normalized transfection efficiency of hMSCs that were untreated, treated with mitomycin C, or treated with gamma radiation. * Denotes significant difference from untreated hMSCs (t test, p<0.05)