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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Crit Care Med. 2012 Apr;40(4):1221–1228. doi: 10.1097/CCM.0b013e31823779f9

Table 2. Michaelis-Menten enzyme kinetic parameter estimates in rat liver microsomes incubated at 37 °C or 33 °C.

Fentanyl Midazolam
37 °C 33 °C 37 °C 33 °C
Vmax (pmol/mg/min) 2480 ± 236 2080 ± 203 2780 ± 60.0 2430 ± 74.8*
Km (μM) 39.8 ± 6.04 38.7 ± 6.15 12.9 ± 0.862 14.1 ± 1.36
Ki (μM) 186 ± 42.0 248 ± 67.5

Vmax, maximum velocity; Km, Michaelis-Menten constant. Ki, dissociation constant for the enzyme-inhibitor complex.

*

Statistically significant differences, p<0.01.

All data are represented as mean±SE.