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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jan 27;1820(4):503–510. doi: 10.1016/j.bbagen.2012.01.011

Fig. 2.

Fig. 2

Minocycline decreases LPS-induced COX-2 mRNA and protein expression, and PGE2 release. Human monocytes were pre-incubated for 2 h with vehicle or minocycline (Mino) and subsequently exposed to 50 ng/ml LPS. COX-2 mRNA expression (A) was quantified by RT-PCR after 4 h of incubation with LPS; COX-2 protein expression (B) and cumulative PGE2 release (C) in monocytes exposed to LPS for 24 h was determined by Western blot and EIA as described in Materials and Methods, respectively. Representative Western blot (B) is shown. Data are presented as means ± SEM from three independent experiments. Statistical significance was determined with one-way ANOVA followed by Newman-Keuls posttest. *P<0.05, ***P<0.001 compared with LPS; ###P<0.001 compared with Saline.