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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Oncogene. 2011 Dec 12;31(36):4045–4053. doi: 10.1038/onc.2011.565

Figure 2. PKCε is a downstream target of miR-107.

Figure 2

A. Ectopic expression of miR-107 reduced PKCε levels in HNSCC. SCC15, SCC25, and CAL27 cells were transfected with premiR-control or pre-miR-107 (50 nmol/L) using Lipofectamine 2000 for 48 hours. Whole cell lysates were extracted and western blot analysis was performed using anti-PKCε and anti-tubulin antibodies. B. Wildtype and mutant miR-107 binding sites in the 3'-UTR of PKCε. C. Luciferase activity controlled by 3'-UTR of PKCε is inhibited by ectopic expression of miR-107. HEK293T cells were co-transfected with wildtype or mutant PKCε-3'UTR and pre-miR-control or pre-miR-107. Renilla and Firefly luciferase activities were measured using the Dual-Luciferase Reporter assay system. Renilla luciferase is normalized to Firefly luciferase and data is presented as mean ± SEM of four independent experiments. *p-value < 0.01.