Skip to main content
. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Oncogene. 2011 Dec 12;31(36):4045–4053. doi: 10.1038/onc.2011.565

Figure 3. Ectopic expression of miR-107 reduces the tumorigenic properties of SCC25 and CAL27 cells in vitro.

Figure 3

SCC25 and CAL27 cells were transfected with pre-miR-control or pre-miR-107 (50 nmol/L) using Lipofectamine 2000. After 48 hours, cells were used for the phenotypic assays. A. Cell proliferation. Cell proliferation was measured using the MTT assay. Data is presented as mean ± SEM. *p-value<0.01, n=3. B. DNA synthesis. Incorporation of 3H-thymidine into DNA was monitored after 4 hours in a scintillation counter. Data is presented as mean ± SEM. *p-value<0.01, n=3. C. Colony formation. Colonies were stained with crystal violet and counted. Data is presented as mean ± SEM. *p-value<0.01, n=3. D. Cell invasion. Cell invasion was assessed using the Modified Boyden chamber invasion assay with Matrigel basement membrane. Invasive cells were counted. Data is presented as mean ± SEM. *p-value<0.01, n=3.