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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Oncogene. 2011 Dec 12;31(36):4045–4053. doi: 10.1038/onc.2011.565

Figure 4. miR-107 reduces tumorigenicity in nude mice.

Figure 4

A. miR-107 expression. Total RNA was isolated from CAL27/miR-control and CAL27/miR-107 cells. miR-107 expression was measured using qPCR. Data is presented as mean ± SEM. *p-value<0.005, n=3. B. Protein levels of miR-107 target genes in miR-107 overexpressing CAL27 cells. Whole cell lysates were extracted and western blot analysis was performed. C. Protein levels of miR-107 target genes in primary keratinocytes transfected with anti-miR-107. Whole cell lysates were extracted and western blot analysis was performed. D. Tumor growth. CAL27/miR-control or CAL27/miR-107 cells were injected subcutaneously to the flanks of the nude mice. Tumors were measured weekly using a caliper and tumor volumes were calculated. Data is presented as mean ± SEM. *p-value<0.005, n=7. E. Tumor weight. Tumors were weighed and data is presented as mean ± SEM for each group. *p-value<0.005, n=7. F. Tumor PKCε levels. Whole cell lysates were isolated from the resected tumors and PKCε levels were measured by western blot analysis.