Table 1.
Neurotransmitter | Primers |
---|---|
Agouti Related Protein (AGRP) | 5′-GCGGAGGTGCTAGATCCA-3′ (forward) 5′-AGGACTCGTGCAGCCTTA-3′ (reverse) 5′-CGAGTCTCGTTCTCCGCG-3′ (probe) |
Neuropeptide-Y (NPY) | 5′-CTCCGCTCTGCGACACTAC-3′ (f) 5′-AATCAGTGTCTCAGGGCT-3′ (r) 5′-CAATCTCATCACCAGACAG-3′ (p) |
Pro-opiomelanocortin (POMC) | 5′-ACCTCACCACGGAGAGCA-3′ (f) 5′-GCGAGAGGTCGAGTTTGC-3′ (r) 5′-TGCTGGCTTGCATCCGGG-3′ (p) |
Thyrotropin-Releasing Factor (TRH) | 5′-CTCTTCTCTCTGACAGCCC-3′ (f) 5′-AGGCGTGGAAGAACCGTC-3′ (r) 5′-TCTCTCGCCCCTTCGACAC-3′ (p) |
Corticotropin-Releasing Hormone (CRH) | 5′-GGAGCCGCCCATCTCTCT-3′ (f) 5′-CCGGGCCATTTCCAAGAC-3′ (r) 5′-ATCTCACCTTCCACCTTCTGCGGGA-3′ (p) |
Ribosomal 18S | 5′-AGTCCCTGCCCTTTGTACACA-3′ (f) 5′-GATCCGAGGGCCTCACTAAAC-3′ (r) 5′-CGCCCGTCGCTACTACCGATTGG-3′ (p) |