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. Author manuscript; available in PMC: 2013 Mar 19.
Published in final edited form as: Chem Res Toxicol. 2012 Feb 21;25(3):730–740. doi: 10.1021/tx200531y

Figure 1.

Figure 1

Running start assays at 37 °C. A preincubated solution of 100 nM 5′-[32P]-labeled 17-mer/26-mer (A, C, E and G) or 17-mer/26-mer-dGAP (B, D, F and H) and 1 μM hPolη (A and B), hPolκ (C and D), hPolτ (E and F), or hRev1 (G and H) was rapidly mixed with a solution of four dNTPs (200 μM each) for various times before being quenched with 0.37 M EDTA. Sizes of important products are denoted and the 21st position marks the location of dGAP from the 3′-terminus of the DNA template.