. Author manuscript; available in PMC: 2013 Mar 19.

Published in final edited form as: Chem Res Toxicol. 2012 Feb 21;25(3):730–740. doi: 10.1021/tx200531y

Table 3.

Binding affinity of hPolη, hPolτ, and hRev1 to normal and damaged DNA at room temperature.

Enzyme	DNA Substrate	With Adduct^a (nM)	Without Adduct^b (nM)	Affinity Ratio^c
hPolη	20-mer/26-mer	7.9 ± 0.3	23 ± 2	3.0
	21-mer/26-mer	8.8 ± 0.5	26 ± 1	2.9
hPolτ^d	20-mer/26-mer	64 ± 8	56 ± 12	0.9
	21-mer/26-mer	ND	43 ± 4	ND
hRev1	20-mer/26-mer	85 ± 5	118 ± 7	1.4
	21-mer/26-mer	ND	ND	ND

With adduct refers to 26-mer-dG^AP.

Without adduct refers to 26-mer.

Calculated as (K_d, _DNA)_Normal/(K_d, _DNA)Damaged.

Values were determined using active site titration assays.

ND denoted ‘not determined’.

All given errors were derived from data fitting.