Table 4.

Kinetic parameters of nucleotide incorporation onto damaged DNA catalyzed by hPolη.

dNTP	Kd, dNTP (μM)	kp (s−1)	kp/Kd, dNTP (μM−1s−1)	Efficiency Ratioa,b	Fidelityc	Fidelity Ratiob,d	Probabilitye (%)
Template dGAP (20/26mer-dGAP)
dCTP	287 ± 24	8.1 ± 0.2	2.8×10−2	20	-	-	77
dATP	634 ± 66	(8.7 ± 0.4)×10−1	1.4×10−3	1.9	4.8×10−2	10	3.9
dGTP	65 ± 7	(5.2 ± 0.2)×10−2	8.1×10−4	1.4	2.8×10−2	14	2.2
dTTP	492 ± 67	3.0 ± 0.2	6.0×10−3	1.1	1.8×10−1	15	17
Template dC (21/26mer-dGAP)
dGTP	125 ± 18	2.2 ± 0.1	1.8×10−2	48	-	-	22
dATP	335 ± 34	(3.3 ± 0.1)×10−1	1.0×10−3	14	5.3×10−2	3.1	1.2
dCTP	119 ± 14	7.3 ± 0.2	6.1×10−2	0.3	7.7×10−1	37	75
dTTP	632 ± 71	(9.5 ± 0.5)×10−1	1.5×10−3	3.1	7.7×10−2	14	1.8

^aCalculated as (k_p/K_d, _dNTP)_normal/(k_p/K_d, _dNTP)_damaged.

^bThe values are from Supplementary Table 1 using control 26mer DNA template.

^cCalculated as (k_p/K_d, _dNTP)_incorrect/[(k_p/K_d, _dNTP)_correct + (k_p/K_d, _dNTP)_incorrect].

^dCalculated as Fidelity_damaged/Fidelity_normal.

^eCalculated as ((k_p/K_d, _dNTP)_damaged/[Σ(k_p/K_d, _dNTP)_damaged])×100.

All given errors were derived from data fitting.