Table 5.

Kinetic parameters of nucleotide incorporation onto damaged DNA catalyzed by hPolκ.

dNTP	Kd, dNTP (μM)	kp (s−1)	kp/Kd, dNTP (μM−1s−1)	Efficiency Ratioa,b	Fidelityc	Fidelity Ratiob,d	Probabilitye (%)
Template dGAP (20-mer/26-mer-dGAP)
dCTP	267 ± 40	1.7 ± 0.1	6.5×10−3	5.7	-	-	71
dATP	497 ± 98	(7.0 ± 0.6)×10−1	1.4×10−3	0.4	1.8×10−1	11	15
dGTP	346 ± 80	(3.4 ± 0.3)×10−1	9.9×10−4	1.1	1.3×10−1	4.5	11
dTTP	726 ± 237	(2.4 ± 0.4)×10−1	3.2×10−4	2.5	4.7×10−2	2.2	3.5
Template dC – pause site (21-mer/26-mer-dGAP)
dGTP	43 ± 7	(2.3 ± 0.1)×10−3	5.3×10−5	208	-	-	60
dATP	203 ± 36	(5.5 ± 0.3)×10−3	2.7×10−5	1.5	3.4×10−1	94	31
dCTP	693 ± 45	(4.1 ± 0.1)×10−3	5.9×10−6	12	1.0×10−1	17	6.7
dTTP	468 ± 90	(12 ± 1)×10−4	2.5×10−6	16	4.5×10−2	13	2.8

^aCalculated as (k_p/K_d, _dNTP)_normal/(k_p/K_d, _dNTP)_damaged.

^bThe values are from Supplementary Table 2 using control 26mer DNA template.

^cCalculated as (k_p/K_d, _dNTP)_incorrect/[(k_p/K_d, _dNTP)_correct + (k_p/K_d, _dNTP)_incorrect].

^dCalculated as Fidelity_damaged/Fidelity_normal.

^eCalculated as ((k_p/K_d, _dNTP)_damaged/[Σ(k_p/K_d, _dNTP)_damaged])×100.

All given errors were derived from data fitting.