Table 6.

Kinetic parameters of nucleotide incorporation onto damaged DNA catalyzed by hPolτ.

dNTP	Kd, dNTP (μM)	kp (s−1)	kp/Kd, dNTP (μM−1s−1)	Efficiency Ratioa,b	Fidelityc	Fidelity Ratiob,d	Probabilitye (%)
Template dGAP (20-mer/26-mer-dGAP)
dCTP	380 ± 66	(8.8 ± 0.6)×10−2	2.3×10−4	7.0	-	-	97
dATP	338 ± 22	(10.3 ± 0.2)×10−4	3.0×10−6	5.0	1.3×10−2	1.4	1.3
dGTP	252 ± 29	(5.3 ± 0.2)×10−4	2.1×10−6	7.6	9.0×10−3	0.9	0.9
dTTP	762 ± 60	(13.1 ± 0.5)×10−4	1.7×10−6	118	7.3×10−3	0.1	0.7
Template dC – pause site (21-mer/26-mer-dGAP)
dGTP	264 ± 76	(5.9 ± 0.6)×10−5	2.2×10−7	4.5×103	-	-	47
dATP	ND	ND	-	-	-	-	-
dCTP	ND	ND	-	-	-	-	-
dTTP	573 ± 73	(14.1 ± 0.8)×10−5	2.5×10−7	680	5.3×10−1	5.5	53

^aCalculated as (k_p/K_d, _dNTP)_normal/(k_p/K_d, _dNTP)_damaged.

^bThe values are from Supplementary Table 3 using control 26mer DNA template.

^cCalculated as (k_p/K_d, _dNTP)_incorrect/[(k_p/K_d, _dNTP)_correct + (k_p/K_d, _dNTP)_incorrect].

^dCalculated as Fidelity_damaged/Fidelity_normal.

^eCalculated as ((k_p/K_d, _dNTP)_damaged/[Σ(k_p/K_d, _dNTP)_damaged])×100.

ND denoted ‘not determined’.

All given errors were derived from data fitting.