Table 7.

Kinetic parameters of nucleotide incorporation onto damaged DNA catalyzed by hRev1.

dNTP	Kd, dNTP (μM)	kp (s−1)	kp/Kd, dNTP (μM−1s−1)	Efficiency Ratioa,b	Fidelityc	Fideltiy Ratioc	Probabilitye (%)
Template dGAP (20-mer/26-mer-dGAP)
dCTP	5.2 ± 0.9	(3.3 ± 0.2)×10−2	6.5×10−3	22	-	-	99.8
dTTP	78 ± 18	(2.6 ± 0.3×10−4	3.4×10−6	7.6×103	5.2×10−4	3.0×10−3	0.1
dATP	103 ± 25	(4.4 ± 0.4×10−4	4.2×10−6	17	6.5×10−41.3	1.3	0.1
dGTP	143 ± 34	(4.2 ± 0.3)×10−4	3.0×10−6	1.8×103	4.6×10−4	0.1	~0

^aCalculated as (k_p/K_d, _dNTP)_normal/(k_p/K_d, _dNTP)_damaged.

^bThe values are from Supplementary Table 4 using control 26mer DNA template.

^cCalculated as (k_p/K_d, _dNTP)_incorrect/[(k_p/K_d, _dNTP)_correct + (k_p/K_d, _dNTP)_incorrect].

^dCalculated as Fidelity_damaged/Fidelity_normal.

^eCalculated as ((k_p/K_d, _dNTP)_damaged/[Σ(k_p/K_d, _dNTP)_damaged])×100.

All given errors were derived from data fitting.