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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Cancer Res. 2012 Jan 12;10(3):282–292. doi: 10.1158/1541-7786.MCR-11-0404

Figure 3. HSCs from osteolytic PCa implanted animals induce osteoclastic colony forming units from BMMCs.

Figure 3

(A) Experimental outline. Co-cultures were established by placing SLAM HSCs (CD150+ Lin− CD48− CD41− Sca-1+ cKit+) derived from animals bearing s.c. tumors or control animals in the top chamber of a dual culture well plate, and mixed mononuclear bone marrow cells in the bottom well. After 2 weeks the phenotype of the resulting colonies were stained for TRAP to visualize osteoclast colonies (CFU-OC). (B) Number of TRAP stained cells/culture. Significant increase in CFU-OCs was noted in the presence of HSCs derived from PC3 tumor-bearing animals vs. surgical control animals. Data represent mean ± SD fold change performed in triplicate in 3 independent cultures. Significance (p<0.001) were calculated compared to surgical control treated group.