Figure 5.
Involvement of reactive oxygen species in PATZ1-induced cellular senescence. Young HUVECs were transfected with PATZ1 siRNA for 4 days and treated with 10 μmol/l dihydrorhodamine 123 for 30 min. (a) Flow cytometric analysis of rhodamine 123 fluorescence. PATZ1 siRNA cells were treated with or without 10 mM N-acetylcysteine for 4 days. (b) Western blotting for p53 and p21CIP1 proteins. (c) SA-β-gal activity staining ( × 100). (d) Percentages of SA-β-gal-positive cells. (e) RT-PCR for MnSOD and catalase. Representative data from three independent experiments are shown. Values are means ±S.D. of three independent experiments. *P<0.05 versus NC, control siRNA; NAC, N-acetylcysteine; siPATZ1, PATZ1 siRNA