Table 3. Effects of mTOR inhibition by rapamycin on miRNA-induced senescence and DDR.

Pre-miRNA	SA-β-gal staining upon rapamycin (% change)a	P-value	γH2AX staining upon rapamycin (% change)b	P-value
Pre-miR-control	−16.2	0.4711	+2.56	0.8520
Pre-miR-210	−43	0.0498	−2.34	0.0808
Pre-miR-376a*	−37.6	0.0052	−2.75	0.2354
Pre-miR-486-5p	−15.3	0.2526	+7.69	0.5734
Pre-miR-494	−46.2	0.0050	−6.59	>0.999
Pre-miR-542-5p	−38.9	0.0262	+6.38	0.9360

^aStarting 24 h after transfection with the indicated miRNAs, cells were treated for 3 days with 10 nM rapamycin or vehicle, and then stained for SA-β-gal. Counts of at least 300 cells were averaged and expressed as percentage of SA-β-gal-stained cells. Reduction in the percentage of positive cells upon rapamycin treatment is reported.

^bStarting 24 h after transfection with the indicated miRNAs, cells were treated for 3 days with 10 nM rapamycin or vehicle, and then examined by immunofluorescence for γH2AX. Counts of at least 300 cells were averaged and expressed as percent of cells with γH2AX foci. Change in the percentage of positive cells upon rapamycin treatment is reported.