Figure 1.

Inhibition of apoptotic cell interaction with THP-1 phagocytes by anti-ICAM-3 mAb MA4. (a) Mutu BL cells were induced to apoptosis by UV (100 mJ/cm²) and co-cultured with THP-1 phagocytes (MØ) in the presence of the indicated mAbs. The proportion of MØ interacting with (tethering and/or phagocytosing) apoptotic cells (ACs) was scored using light microscopy following Jenner/Giemsa staining. Data (mean±S.D.) from at least three separate experiments each carried out in quadruplicate are shown (data are normalised to the mAb-free co-culture (‘Alone'). (b) Microscopic appearance of a THP-1 phagocyte culture or co-cultures of THP-1 and ACs in the presence or absence of the indicated mAb (MA4 or BH10). Scale bar=50 μm. ^**P<0.01 ANOVA with Dunnett's post-hoc test