Figure 3.

Tethering (binding) of apoptotic cells to human or mouse phagocytes is reduced in the absence of apoptotic cell ICAM-3. BL and Jurkat cells (WT or ICAM-3 deficient) were induced to apoptosis by UV (Mutu: 100 mJ/cm²; Jurkat 150 mJ/cm²). The capacity of THP-1 phagocytes to tether apoptotic cells (WT, grey bars; ICAM-3-deficient, open bars) was assessed at room temperature (nonpermissive for phagocytosis) and compared with interaction (binding and phagocytosis), which was assessed at 37°C (a). Similar tethering assays using J774 and RAW mouse phagocytes are shown (b). Data shown are mean±S.D. of replicate samples (n=4) from one experiment of at least three similar. ^**P<0.01; ^***P<0.001 ANOVA with Tukey's post-hoc test