Figure 4.

Immunodominance is not solely determined by TCR affinity for MHC–peptide complexes. Dispersed TG were stained with gB_498–505 or RR1_982–989 tetramer for 1 h at 37°C. Anti–H-2K^b/H-2D^b blocking Ab was added, and TG were incubated for the designated times at 37°C to observe tetramer dissociation. Data represent the mean fluorescence intensity (MFI) of CD8⁺ tetramer⁺ cells as a percentage ± SEM (n = 3) of the maximum MFI observed at time zero. Linear regression represents the slopes of both gB_498–505 and RR1_982–989. Data are representative of three independent experiments.