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. Author manuscript; available in PMC: 2012 Mar 20.
Published in final edited form as: Cell Cycle. 2009 Jun 3;8(11):1738–1746. doi: 10.4161/cc.8.11.8619

Figure 5.

Figure 5

Hypercholesterolemia is associated with increased phosphorylation of mTORC2 targets, and an increase in PKCα levels in the myocardium. Representative western blots for phospho-Akt (Ser473), phospho-PKC (α, Ser657), total PKCα and α-tubulin in the left ventricle of hypercholesterolemic (HC) and normocholesterolemic (NC) pigs, and quantification of the data. For total levels of Akt corresponding to the phospho-Akt (Ser473) blot, see Figure 2. The phospho-PKC antibody was a phospho-pan PKC antibody with reactivity to PKCα when phosphorylated at Ser657. This is the thicker bottom band which overlapped with total PKCα. Bands were rearranged from the original gel for clarity of presentation. Densities were calculated relative to total Ponceau S staining. Phosphorylation is presented as the phospho-to-total (p/t) ratio of the protein. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 versus control.