Figure 7.
Hypercholesterolemia is associated with increased levels of hypertrophy-associated proteins, and a trend toward decreased autophagy in the myocardium. Representative western blots for β-actin, troponin I, troponin T, SERCA2 ATPase, α-tubulin, LC3B-I&II and GAPDH as a loading control in the left ventricle of hypercholesterolemic (HC) and normocholesterolemic (NC) pigs, and quantification of the data. Bands were rearranged from the original gel for clarity of presentation. Densities were calculated relative to total Ponceau S staining. The ratio of LC3B-II to I (LC3B-II/Ir) was calculated by dividing the density of the bottom band by that of the top on the LC3B blots. Data are expressed as mean ± SEM. *p < 0.05 versus control.