Figure 7.
In vivo differentiation of low-dose hUCB-MSCs in the ischemic brain. Confocal images of the cells at four weeks after 5 × 105 hUCB-MSC administration in the ischemic animal models. hUCB-MSCs were identified by the staining with human nuclei antibody (hNA, green). A subset of the grafted cells expressed (a) NeuN and (b) GFAP in the ipsilateral ischemic boundary zone. These markers were immunolabeled with red fluorescence. Quantitative analysis of (c) hNA-NeuN and (d) hNA-GFAP double-positive cells in the ipsilateral IBZ. Data are presented as mean values ± SD. There were significantly more double-positive cells in animals after intrathecal administration (analysis of variance; *P < 0.05). Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm.