Figure 6. Evaluation of microvessel density in Lewis lung carcinoma tumours.

A) Immunohistochemical evaluation of microvessels in Lewis lung tumours using antibodies to PECAM-1 as described in the Methods section. The tumours were treated in vivo with fisetin or cyclophosphamide alone, and with the combination of cyclophosphamide and fisetin. Scale bar, 100 μm. B) Microvessel density (number of vessels per mm²) in tumours after in vivo treatment with the solvent alone (control), with fisetin or cyclophosphamide (CPA) alone, or with the combination of fisetin and cyclophosphamide (CPA). Mean ± SEM. The asterisks indicate a P value < 0.05 compared to controls (Dunnett’s multiple comparison test).