Fig. 5.

Distribution of Slit mRNA in the cervical spinal cord. (A-D) Serial cryosections derived from E10.5 WT embryos were labeled with Slit1 (A), Slit2 (B) or Slit3 (C) riboprobes. (D) Pseudo-color image representing the merge of A-C. High levels of Slit2 (B,D, arrowheads) and low levels of Slit1 and Slit3 are expressed at the LEP (A,C,D). High-magnification micrographs depicting LEP-associated expression are included as insets (A-C, arrowheads). Dotted lines demarcate spinal cord margin. Scale bar: 50 μm.