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Grg3 expression is lost in Ngn3–/– pancreas. (A,B) Grg3 protein is expressed in E15.5 Ngn3+/+ pancreata (A, arrows) but is lost in Ngn3–/– (B). (C,D) Positive control for antibody staining shows Pdx1 staining in the pancreas epithelium of Ngn3+/+ and Ngn3–/– pancreata.