Fig. 7.

The different expression patterns of Ndg in various mesodermal cell types involve both different Fkh TFs and distinct modes of use of Fkh-binding sites in the Ndg enhancer. (A) Summary of: (1) the effects of mutating different Fkh binding sites in the Ndg enhancer; and (2) the effects of loss of function of bin, jumu and CHES-1-like on the activity of the wild-type Ndg enhancer in different types of mesodermal cells. (B) In the VM, the convergence of cis, trans and EMSA data suggest that Bin binds either to both Fkh1 and Fkh2 sites or to both Fkh1 and Fkh3 sites to activate Ndg expression. (C) In somatic myoblasts, the cis, trans and EMSA results are consistent with CHES-1-like binding to the Fkh2 or Fkh3 sites to repress Ndg reporter expression in FCMs. The contribution, if any, of CHES-1-like binding at the Fkh1 site to the repression of the Ndg reporter could not be determined by the design of the experiment and is denoted by ‘?’ in the panel. (D,E) CHES-1-like binds to all three sites, whereas Jumu binds to the Fkh2 site to repress Ndg reporter expression in CCs and Odd-PCs.