Figure 4.

Quantitative assessment of the impact of D/R on the binding affinities of different antibodies, specifically captured by chemically immobilized RAMFc [36], to HPV16 VLPs by SPR. (A) Enhanced binding of neutralizing mAbs (V5, E70) and greatly reduced binding of linear epitope targeting mAbs (J4, O7 and H5) upon VLP reassembly. (B) Relative footprint changes of anti-HPV 16 L1 mAbs and their approximate degree of overlapping in pre- and post-D/R samples-data obtained with pair-wise epitope mapping.