Fig. 8.

Expression of Edn1 depends on epithelial β-catenin. A–D: Whole-mount RNA in situ hybridization using Edn1 specific probe. white arrowhead, normal expression in mandibular prominence (md); red arrowhead, reduced expression in mutants. expression in hyoid arch (hy) and the rest of caudal arches are unaffected. E: Real-time quantitative polymerase chain reaction analyses of relative expression levels of genes (x-axis) in the microdissected first pharyngeal arch at embryonic day (E) 10.5 using β-actin as an internal control. Asterisk, P < 0.05 (y-axis), Student t-test, n = 4.