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. 2012 Mar 19;196(6):801–810. doi: 10.1083/jcb.201112098

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© 2012 Roux et al.

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Figure 4. — SLAP75 is a novel peripheral membrane constituent of the NE identified with BioID-LaA. To determine if FAM169A/SLAP75 is a novel peripheral membrane constituent of the NE its subcellular localization was analyzed by immunofluorescence microcopy. (a) As detected with anti-SLAP75 (red), the endogenous protein is colocalized with LaA (green) at the NE of HEK293 cells. (b) Although endogenous SLAP75 is not detected in HeLa cells (not depicted), transiently expressed HA-SLAP75 (red) detected with anti-FAM169A/SLAP75 is localized to the NE, labeled with anti-Nup153 (green). DNA was labeled with Hoechst (blue). Bar, 10 µm.