TABLE 2.

LC-MS/MS identification of proteins purified by Tb11.01.4590 PTP pulldown assays

TM, transmembrane.

Gene ID	Protein ID	Pulled down by tRNA?	Predicted MLS or TMa	Domain homologyb	PF/BF mRNA expressionc
Tb927.8.1740	Hypothetical	Yesd	Mito	None	Yes
Tb11.01.4870	Tim17	No	Both	Protein import	Yes
Tb927.3.3330	HSP20	Yesd	Mito	Chaperone	Yes
Tb927.6.1680	Hypothetical	Yesd	Mito	C2H2-zinc finger	Yes
Tb927.10.6400	HSP60	Yes	Mito	Protein import	Yes
Tb11.03.0475	Hypothetical	Yesd	Mito	None	Yes
Tb927.6.3740	HSP70	Yes	Mito	Protein import	Yes
Tb11.02.3310	Hypothetical	Yesd	Both	None	Yes
Tb09.211.2530	Hypothetical	No	Both	None	Yes

^a Predictions based on sequence analysis using MitoProt/TMHMM/TMPred software and Panigrahi et al. (40).

^b Domain homology by BLASTp analysis through NCBI/CDD.

^c Life stage expression data from Nilsson et al. (44).

^d At least one peptide >95% confidence identified by tRNA pull down MS/MS analysis.