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Identification of phosphorylated residues by phosphate affinity SDS-PAGE. A and C. U2OS cells were transfected with Myc-YAP mutant constructs depicted in B. Cell lysates were resolved on the gel containing 25 μm of Phos-tag ligand and blotted with antibodies indicated. B, YAP point mutants analyzed in this study are summarized. The residues at the indicated positions were changed to Ala. The mutated residues in YAP 4A are illustrated with the position of domains.
