FIGURE 7.

Regulation of apoptosis by YAP phosphorylation mutants. A and B, KB cells expressing Tet-on shYAP were incubated in the presence or absence of Dox for 72 h and then treated with CDDP (15 μm) for the periods indicated. Cell lysates were analyzed by immunoblotting with indicated antibodies (A). At 18 h after CDDP treatment, the extent of apoptotic cell death was quantified by the percentage of cells showing subdiploid DNA content by FACS analysis (B). PI, propidium iodide. C and D, KB cells expressing Tet-on shYAP were restored with shRNA-resistant YAP constructs. Cells were treated and analyzed as in A, and the extent of apoptotic cell death was analyzed as in D.