TABLE 1.

Summary of ATP hydrolysis and nucleotide binding data of HsRAD51 wild type and HsRAD51(D316K) mutant protein

Kinetic parameter	WT	D316K
kcat (min−1)
ssDNA	0.180 ± 0.004	0.070 ± 0.005
ssDNA, KCI	0.190 ± 0.004	0.063 ± 0.005
dsDNA	0.167 ± 0.002	0.060 ± 0.007
dsDNA, KCI	0.135 ± 0.007	0.052 ± 0.005
Km (μm)
ssDNA	17.48 ± 3.25	3.08 ± 0.10
ssDNA, KCI	9.34 ± 1.42	2.14 ± 0.002
dsDNA	6.54 ± 0.67	2.17 ± 0.15
dsDNA, KCI	5.52 ± 0.26	1.70 ± 0.003
KD (μm) ATPγS
ssDNA	2.49 ± 0.46	0.66 ± 0.21
ssDNA, KCI	1.18 ± 0.22	0.72 ± 0.21
Bmax (μm) ATPγS
ssDNA	1.02 ± 0.02	0.90 ± 0.03
ssDNA, KCI	0.83 ± 0.04	0.87 ± 0.05
KD (μm) ADP
ssDNA, KCI	1.24 ± 0.32	0.26 ± 0.06
Bmax (μm) ADP
ssDNA, KCI	0.82 ± 0.05	0.86 ± 0.02