FIGURE 1.

Assay performance and Z′-factors of the coupled peroxidase assay. a, peroxidase activity was measured by following NADPH consumption at 340 nm with six reads every 19 min. A reaction mixture lacking Px represents 100% inhibition (positive control), whereas one containing Px but no inhibitor corresponds to 0% inhibition (negative control). The percentage of inhibition was calculated from the respective slopes as exemplified here for compound 6 (gray circles), which resulted in an inhibition of 65%. b, the full screening approach comprised 78,637 compounds distributed in 246 plates (384-well plates). The Z′-factor was calculated as described under “Experimental Procedures.”