. 2012 Jan 23;287(12):8792–8802. doi: 10.1074/jbc.M111.338285

TABLE 3.

Identification of the target protein(s)

Peroxidase activity was measured as described under “Experimental Procedures.” The reaction was started either directly or after 40 min preincubation of the reaction mixture (complete, without either Px or Tpx) by adding the missing protein component immediately followed by H₂O₂. Values are the means ± S.D. of three assays. ND means not determined.

Compound	Inhibition
	No Preincubation, complete		Preincubation, complete		Preincubation-Px	Preincubation-Tpx
	Px	Prx	Px	Prx	Px	Px
	%
1	29 ± 4^a	40 ± 3^b	84 ± 1^a	ND	87 ± 1^a	13 ± 2^a
2	3 ± 1^a,d	9 ± 0.2^c,d	85 ± 4^a	ND	21 ± 4^a	3 ± 1^a
3	29 ± 1^c	ND	102 ± 2^c	102 ± 2^c	100 ± 1^c	21 ± 2^c
4	3 ± 1^c	ND	78 ± 3^c	75 ± 2^c	90 ± 1^c	11 ± 3^c
5	10 ± 2^c	ND	93 ± 1^c	ND	100 ± 1^c	13 ± 1^c
6	97 ± 1^a	88 ± 2^a	105 ± 0.3^a	ND	99 ± 2^a	70 ± 2^a
9	2 ± 0.4^c	ND	75 ± 1^c	85 ± 2^c	78 ± 1^c	10 ± 3^c
10	84 ± 3^a	74 ± 3^a	99 ± 2^a	ND	96 ± 1^a	6 ± 4^a
11	71 ± 1^a	64 ± 1^a	102 ± 1^a	ND	101 ± 2^a	12 ± 2^a
12	19 ± 3^c	ND	101 ± 2^c	102 ± 1^c	99 ± 1^c	17 ± 2^c

^a The assay contained 10 μm of the respective inhibitor.

^b The assay contained 20 μm of the respective inhibitor.

^c The assay contained 40 μm of the respective inhibitor.

^d The values refer to the activity calculated for the 1st min. In the presence of compound 2, NADPH oxidation declined rapidly during the course of the assay. This resulted after 3 min in 32% and 45% inactivation in the Px and Prx assay, respectively.