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Western blot detection of native and recombinant Bc28.1 protein by monoclonal antibody. A, Coomassie-stained SDS-PAGE of the samples analyzed by Western blot. B, Western blots probed with hybridoma supernatant from monoclonal antibody 6C9 or with culture medium alone. Lane 1, ghosts from dog erythrocytes; lane 2, ghost from dog erythrocytes parasitized by B. canis; lane 3, recombinant Bc28.1 protein; lane 4, recombinant His-GST protein.
