FIGURE 6.
membrane-bound and soluble forms of Bc28.1 immunoprecipitated from fractionated samples. A, [35S]methionine-labeled, parasitized dog erythrocytes were fractionated in purified merozoites, soluble fraction, and ghosts and then immunoprecipitated either with preimmune serum (lanes 1) or rabbit anti-Bc28.1 serum (lanes 2). B, purified merozoites and soluble fraction were submitted to Triton X-114 phase partitioning. The detergent-enriched phase (Det.) and aqueous phase (Aq.) were then immunoprecipitated using anti-Bc28.1 serum.