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. 2012 Jan 31;287(12):9495–9508. doi: 10.1074/jbc.M111.260745

FIGURE 7.

FIGURE 7.

Bc28.1 is an erythrocyte-binding protein from B. canis. A, binding assay performed with increasing amounts of dog erythrocytes (PCV, packed cell volume ranging from 10 to 100 μl). His-GFP is used as a negative control. Upper gel, unbound proteins; lower gel, bound proteins revealed by anti-His tag. B, binding assay with increasing amounts of recombinant Bc28.1 and a constant amount (50 μl) of packed red blood cells. His-GFP is used as a negative control. Upper gel, unbound proteins; lower gel, bound proteins revealed by anti-His tag.