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. 2012 Jan 19;287(12):9147–9167. doi: 10.1074/jbc.M111.316554

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — A, Western blot analysis using anti-FLAG and anti-SP-STP antibodies to depict the expression of STP-FLAG (Panel I) and SP-STPD192A (Panel II) 72 h post-transfection in Detroit 562 cell lysates. B, MTT assay depicting the proliferation index of the Detroit 562 cells transfected with pCMV-FLAG (vehicle control), pCMV.stp-FLAG, and pCMV-stpD192A.FLAG at the indicated time points. C, confocal microscopic differential interference contrast (DIC) and merged images showing cytoplasmic localization of GFP (24 h) and nuclear localization of SP-STP-GFP (12 and 24 h). Nucleus is stained with Hoechst33342. Blue DAPI was changed to the red channel for clarity of the superimposed images.