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. 2012 Jan 24;287(12):9269–9279. doi: 10.1074/jbc.M111.316208

FIGURE 5.

FIGURE 5.

Increased interaction of an Rpt dimer containing a Walker A mutant with the adjacent dimer by S5b and PAAF1. A, immunoblotting analysis of wild-type and Walker A mutant FLAG-Rpt1 samples following expression of S5. HeLa cells were transfected with the expression constructs of wild-type or Walker A mutant FLAG-Rpt1 along with HA-tagged Rpt2, Rpt4, Rpt5, and S5B. Immunopurified FLAG-Rpt1 samples were analyzed by immunoblotting with indicated antibodies. B, immunoblotting analysis of wild-type and Walker A mutant FLAG-Rpt6 samples following the expression of PAAF1. Hela cells were transfected with the expression constructs of wild-type or Walker A mutant FLAG-Rpt6 along with HA-tagged Rpt1, Rpt2, Rpt3, and PAAF1. Immunopurified FLAG-Rpt6 samples were analyzed by immunoblotting with the indicated antibodies.