FIGURE 7.

Impaired incorporation of mutant Rpt3 with a deletion in the HbYX motif into the proteasome. A and B, immunoblotting analysis of the proteasome following transfection of wild-type or C-terminal three-residue deletion mutant Rpt3 (A) or Rpt6 (B). HeLa cells expressing FLAG-Rpn12 were transfected with the expression constructs of HA-tagged wild-type or C-terminal deletion mutant Rpt3 or Rpt6. After affinity purification of the proteasome in the presence of 5 mm ATP, the samples were analyzed by immunoblotting with the indicated antibodies.