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. 2012 Jan 25;287(12):8920–8931. doi: 10.1074/jbc.M111.327411

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — DUG2 and DUG3 are under sulfur regulation. A, C, and D, β-galactosidase reporter assay plasmid (pLG699Z) having a 600-bp upstream region of DUG3, fused upstream of the lacZ gene, was transformed in MET15 (BY4742) and met15Δ (BY4741) strains (A), met4Δ strains (C), and cbf1Δ and met28Δ strains (D). B and E, β-galactosidase reporter assay plasmid (pLG699Z) having a 1-kb upstream region of DUG2, fused upstream of lacZ gene, was transformed in met15Δ strains (B) and met28Δ strains (E). Transformants were grown under different sulfur sources (-S, 0 μm methionine; +Met, 200 μm methionine; +GSH, 200 μm glutathione). β-Galactosidase activity is assayed for each condition as described under ”Experimental Procedures.“ The experiment was repeated twice in duplicate, and mean ± S.E. is plotted.