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Fluorescence quenching analyses of capacity of siderocalin to bind ferric enterobactin (FeEnt), vibriobactin (Vb), and ferric vibriobactin (FeVb). Fluorescence data were taken at 340 nm (pH 7.4) upon the addition of substrates to a 200 nm solution of siderocalin. Data points indicate the fluorescence signals generated by siderocalin, and curves indicate the calculated fits.
